Novus Biologicals™ Mouse TNF-alpha ELISA Kit (Colorimetric)

Description

ELISA kits are ideal for the quantification of target antigens in tissue extracts, serum and cell culture.

The Mouse TNF-alpha ELISA Kit (Colorimetric) measures mouse TNF-alpha in cell culture supernatants, plasma (edta, citrate), serum.

Specifications

• Assay Range: 31.3 to 2000 pg/mL
• Assay Sensitivity: 3.7pg/mL
• Product Type: ELISA Kit
• Specificity: The assay detects both natural and recombinant mouse TNF alpha. The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into mouse IL-10 positive serum. There was no crossreactivity detected.
• Target Species: Mouse
• For Use With (Application): ELISA
• Gene ID (Entrez): 7124
• Gene Symbol: TNF
• Kit Contents: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse TNF-alpha, Biotin-Conjugate anti-mouse TNF-alpha polyclonal antibody, Streptavidin-HRP, Mouse TNF-alpha Standard lyophilized, 2ng/mL upon reconstitution, Calibrator Diluent, Sample Diluent, Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA), Wash Buffer Concentrate 20x (PBS with 1% Tween 20), Substrate Solution (tetramethyl-benzidine), Stop Solution (1M Phosphoric acid), Adhesive Films
• Quantity: 96 Tests
• Research Discipline: Adaptive Immunity, Asthma, Autophagy, Biologically Active Proteins, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Storage Requirements: Storage of components varies. See protocol for specific instructions.
• Synonym: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necr
• Target: TNF-alpha