MP Biomedicals™ Beta-Galactosidase from Escherichia coli Greater than 300U/mg

Description

Useful for the structural investigation of carbohydrate, the determination of lactose and as an enzyme label for enzyme immunoassay

β-Galactosidase cleaves terminal galactose residues, which are β -1-->14 linked to a monosaccharide, glycopeptide, or oligosaccharide. The enzyme requires Mg₂+ for optimal activity.

• Substrate Specificity: Enzyme specifically hydrolyzes β-D-galactosyl linkage
• Presentation: White powder
• Isoelectric point: 4.61 (Lit.)
• Optimum pH: 7.0 to 7.5, pH
• Stability: pH 6.5 to 8.5 (25°C, 20 hr)(Lit.); Thermal Stability: Below 50°C (pH 7.3, 15 min) (Lit.)
• Optimum Temperature: 50 to 55°C (Lit.)
• Unit Definition: One unit catalyzes the hydrolysis of 1 μmole of o-nitrophenyl-β-D-galactopyranoside per minute at 25°C, pH 7.5

Specifications

• Concentration: ≥300 U/mL
• Color: White
• Components: Reaction mixture- 2.5mL 0.1M Phosphate Buffer (pH 7.3), 0.1mL Mercaptoethanol solution, 0.1mL MgCl2 solution , 0.2mL ONPG solution
• pH: 7 to 7.5
• For Use With (Application): Structural investigation of Carbohydrate, the determination of Lactose (food stuff analysis) and as an Enzyme label for Enzyme immunoassay
• Content And Storage: 0°C
• Source: E. coli
• Quantity: 1 kU
• Product Type: beta-Galactosidase
• Molecular Weight (g/mol): 540000
• Inhibitors: p-Chloromercuribenzoate, lodoacetamide, Heavy Metal Ions (Zn++, Fe++, Zn++, Cd++, Cu++, Pb++, Ag+, Hg++), Ionic Detergents SDS, DAC
• Form: Solid