mPEG, Mouse anti-All, Clone: 15-2b, Millipore Sigma™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABS200225	MilliporeSigma MABS200225UG	25 μg
MABS2002100	MilliporeSigma MABS2002100UG	100 μg

Description

Anti-mPEG, clone 15-2b, Cat. No. MABS2002, is a mouse monoclonal antibody that detects methoxy-PEG (mPEG)-conjugated proteins and has been tested for use in ELISA.

Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solutions. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. The monomethoxylated form of polyethylene glycol (mPEG) has been widely employed in drug modifications. mPEG modification reduces nonspecific binding and prevents recognition by the reticuloendothelial system. This monoclonal antibody (clone 15-2b) specifically binds to the terminal methoxy group, but not the backbone of PEG. It displays high affinity for the terminal methoxy group and can be easily used as a detection antibody for the quantification of mPEG by ELISA. It is also suitable for detection of PEGylated liposomes and nanoparticles. The mPEG cell-based competition ELISA can detect lower molecular-weight (< 2 kDa) mPEG molecules even at nanomolar concentration and the assay is not affected by the presence of mouse or human serum. In addition, mPEG cell-based ELISA can be useful in determining the pharmacokinetics of a mPEG molecule an animal without the need for any radiolabeling.

Specifications

• Antigen: mPEG
• Applications: ELISA
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Signaling
• Formulation: Purified mouse monoclonal antibody IgG2b in PBS with 0.04% sodium azide.
• Immunogen: Methoxy PEG covalently conjugated to E.coli beta-glucuronidase.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.

• Clone: 15-2b
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: All species
• Gene Alias: Methoxy polyethylene glycol
• Isotype: IgG2b κ
• Purification Method: Protein A purified
• Test Specificity: Clone 15-2b specifically detects proteins conjugated with methoxy polyethylene glycol (mPEG).