MR1, Mouse anti-Rat, Human, Mouse, Bovine, Clone: 26.5, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABF207525UL

Catalog No. MABF207525

Description

Anti-MR1, clone 26.5, Cat. No. MABF2075, is a mouse monoclonal antibody that detects MHC class I-related gene protein and has been tested for use in Flow Cytometry, Function Analysis, and Immunoprecipitation.

Major histocompatibility complex class I-related gene protein (UniProt: Q95460; also known as MHC class I-related gene protein, Class I histocompatibility antigen-like protein, MR1) is encoded by the MR1 gene (Gene ID: 3140) in human. MR1 is a single-pass membrane protein that is ubiquitous in its distribution and can heterodimerize with B2M. It serves as an antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. It is involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain known as mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and may be involved in monitoring commensal flora or serve as a distress signal. MR1-restricted T cells can discriminate between microbes in a TCR-dependent manner. However, these cells are shown to be severely reduced in circulation in subjects with chronic HIV-1 infection. MR1 is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. The mature form has an extracellular domain (aa 23-302), a transmembrane domain (aa-303-323), and a short cytoplasmic tail (aa 324-341). Its ligand binding region is localized to amino acids 23-201. Five different isoforms of MR1 have been described that are produced by alternative splicing. (Ref.: Young, MH et al., (2013). PLOS one 8(1); e53789; Leeansyah, E., et al. (2013). Blood. 121(7); 1124-1135).

Specifications

• Antigen: MR1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG2a in PBS without azide.
• Gene Symbols: MR1
• Immunogen: GST-tagged recombinant fragment corresponding to 280 amino acids from the extracellular domain of human MHC class I-related gene protein.
• Quantity: 25 μL
• Research Discipline: Inflammation & Immunology
• Gene ID (Entrez): NP_001522.1
• Target Species: Rat, Human, Mouse, Bovine
• Form: Purified

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Clone: 26.5
• Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated MR1 in Immunoprecipitation applications (Huang, S., et. al. (2005). J Biol Chem. 280(22):21183-93).Flow Cytometry Analysis: A representative lot detected MR1 in flow cytometry applications (Huang, S., et. al. (2005). J Biol Chem. 280(22):21183-93; Gold, M.C., et. al. (2010). PLoS Biol. 8(6):e1000407; Huang, S., et. al. (2009). Proc Natl Acad Sci U S A. 106(20):8290-5). Inhibits Activity/Function Analysis: A representative lot inhibited the activation of mucosal-associated invariant T (MAIT) cells by MR1 protein. (Huang, S., et. al. (2009). Proc Natl Acad Sci U S A. 106(20):8290-5).
• Gene Alias: Major histocompatibility complex class I-related gene protein;MHC class I-related gene protein;Class I histocompatibility antigen-like protein
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 26.5 specifically detects Major histocompatibility complex class I-related gene protein (MR1). It targets an epitope with in the extracellular domain.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG2a κ