MUC5AC Antibody (2-11M1), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2444580.1MG

Catalog No. NBP24445801

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence

MUC5AC Monoclonal specifically detects MUC5AC in Human, Mouse, Bovine, Feline, Monkey samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence.

Specifications

• Antigen: Mucin 5AC
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
• Gene Alias: gastric mucin, leB, lewis B blood group antigen, major airway glycoprotein, MUC5, mucin 5, subtypes A and C, tracheobronchial/gastric, mucin 5AC, oligomeric mucus/gel-forming, mucin 5AC, oligomeric mucus/gel-forming pseudogene, mucin-5 subtype AC, tracheobronchial, mucin-5AC, TBM, tracheobronchial mucin
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb 2-11M1 reacts with the protein backbone exclusively; it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then 2-11M1 will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), SDS-Page, Immunofluorescence
• Clone: 2-11M1
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: MUC5AC
• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient.
• Quantity: 0.1 mg
• Research Discipline: Extracellular Matrix
• Gene ID (Entrez): 4586
• Target Species: Human, Mouse, Bovine, Feline, Monkey
• Form: Purified

For Research Use Only