Myeloperoxidase/MPO Antibody (2C7), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NB100648030.025MG

Catalog No. NB017646

Description

Myeloperoxidase/MPO Monoclonal specifically detects Myeloperoxidase/MPO in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Myeloperoxidase/MPO
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:500-1:1000, Immunohistochemistry-Frozen 1:1000-1:5000
• Gene Accession No.: P05164
• Gene Symbols: MPO
• Immunogen: Human Myeloperoxidase
• Quantity: 0.025 mg
• Research Discipline: Cell Biology, Immunology, Innate Immunity, Lipid and Metabolism
• Gene ID (Entrez): 4353
• Target Species: Human
• Form: Purified

• Applications: Immunohistochemistry (Frozen), Flow Cytometry, ELISA, Western Blot, Immunohistochemistry (Paraffin), Immunohistochemistry
• Clone: 2C7
• Conjugate: Unconjugated
• Formulation: Phosphate buffered saline with 0.09% Sodium Azide
• Gene Alias: EC 1.11.1, EC 1.11.1.7, myeloperoxidase
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1

For Research Use Only