Myeloperoxidase/MPO Antibody (2C7), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB017646	Novus Biologicals NB100648030.025MG	0.025 mg
NB10064803	Novus Biologicals NB10064803	0.1 mg

Description

Myeloperoxidase/MPO Monoclonal specifically detects Myeloperoxidase/MPO in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Myeloperoxidase/MPO
• Concentration: 1 mg/mL
• Applications: Immunohistochemistry (Frozen), Flow Cytometry, ELISA, Western Blot, Immunohistochemistry (Paraffin), Immunohistochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cell Biology, Immunology, Innate Immunity, Lipid and Metabolism
• Gene Accession No.: P05164
• Gene ID (Entrez): 4353
• Immunogen: Human Myeloperoxidase
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: 2C7
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:500-1:1000, Immunohistochemistry-Frozen 1:1000-1:5000
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: EC 1.11.1, EC 1.11.1.7, myeloperoxidase
• Gene Symbols: MPO
• Isotype: IgG1
• Purification Method: Protein G purified
• Test Specificity: NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.

For Research Use Only