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MyoD Mouse, Clone: SPM427, Novus Biologicals™
SDP

Catalog No. NBP232882F Shop All R&D Systems Products
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NBP232882F 0.02 mg
NBP232882A 0.1 mg
NBP232882B 0.2 mg
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Catalog No. NBP232882F Supplier Novus Biologicals Supplier No. NBP2328820.02MG
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Description

Mouse Monoclonal Antibody has been used in 2 publications

MyoD Monoclonal specifically detects MyoD in Human, Mouse, Rat, Chicken samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

Antigen MyoD
Applications Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone SPM427
Conjugate Unconjugated
Dilution Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Formulation PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Accession No. P15172
Gene Alias bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
Gene Symbols MYOD1
Host Species Mouse
Immunogen Recombinant mouse MyoD1 protein
Molecular Weight of Antigen 45 kDa
Purification Method Protein A purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cancer, Cell Cycle and Replication, Growth and Development, Phospho Specific
Primary or Secondary Primary
Gene ID (Entrez) 4654
Test Specificity Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.
Target Species Human, Mouse, Rat, Chicken
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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