MyoD Mouse, Clone: SPM427, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Catalog Number	Mfr. No.	Quantity
NBP232882F	Novus Biologicals NBP2328820.02MG	0.02 mg
NBP232882A	Novus Biologicals NBP2328820.1MG	0.1 mg
NBP232882B	Novus Biologicals NBP2328820.2MG	0.2 mg

Description

MyoD Monoclonal specifically detects MyoD in Human, Mouse, Rat, Chicken samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

• Antigen: MyoD
• Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Chicken
• Gene Accession No.: P15172
• Gene ID (Entrez): 4654
• Immunogen: Recombinant mouse MyoD1 protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 45 kDa

• Clone: SPM427
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Cycle and Replication, Growth and Development, Phospho Specific
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
• Gene Symbols: MYOD1
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.