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Myogenin Antibody (MGN185), DyLight 755, Novus Biologicals™
SDP

Catalog No. NBP233056IR Shop All R&D Systems Products
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Catalog No. NBP233056IR Supplier Novus Biologicals Supplier No. NBP233056IR
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Description

Mouse Monoclonal Antibody

Myogenin Monoclonal specifically detects Myogenin in Human, Mouse, Rat, Porcine, Feline samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

Antigen Myogenin
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Monoclonal
Clone MGN185
Conjugate DyLight 755
Dilution Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Gene Alias BHLHC3, bHLHc3Myogenic factor-4; myogenin, Class C basic helix-loop-helix protein 3, myf-4, MYF4myogenin, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4)
Gene Symbols MYOG
Host Species Mouse
Immunogen Human Myogenin recombinant protein (Uniprot: P15173)
Molecular Weight of Antigen 34 kDa
Purification Method Protein A or G purified
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Cancer, Stem Cell Markers, Transcription Factors and Regulators
Primary or Secondary Primary
Gene ID (Entrez) 4656
Test Specificity Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
Target Species Human, Mouse, Rat, Pig, Feline
Content And Storage Store at 4C in the dark.
Form Purified
Isotype IgG1 κ
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