Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-8752	Invitrogen™ 53650380	25 μg
50-112-8865	Invitrogen™ 53650382	100 μg

Description

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis. The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog. Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections. Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.

Specifications

• Antigen: Myosin 4
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: 2B myosin heavy chain; AI506973; fast myosin heavy chain 2B; LOW QUALITY PROTEIN: myosin-4; MHC; MHC2B; Minimsc; Minmus; MM; MYH1; MYH2B; MYH-2B; myh4; MyHC IIb isoform; myHC-2b; MyHC-IIb; Myhsf; myosin heavy chain; myosin heavy chain 1; myosin heavy chain 2b; myosin heavy chain 2X; myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain IIb isoform; myosin heavy chain isoform 2b; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; myosin-4; unnamed protein product
• Gene Symbols: MYH4
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: MYH4

• Clone: MF20
• Applications: Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Conjugate: Alexa Fluor 488
• Host Species: Mouse
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Monkey, Bovine, Feline, Chicken, Guinea Pig, Chimpanzee
• Gene Accession No.: Q076A5, Q28641, Q29RW1, Q5SX39, Q9Y623
• Gene ID (Entrez): 100009548, 100125991, 100726456, 17884, 360543, 408020, 4622, 468396, 479502
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.