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PeproTech NAMPT Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No. p-7245474
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25 μg
50 μg
500 μg
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Catalog No. Quantity
500P222BT25 25 μg
500P222BT50 50 μg
50-271-8599 500 μg
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Catalog No. 500P222BT25 Supplier PeproTech Supplier No. 500P222BT25UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Visfatin. Anti-Human Visfatin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human Visfatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Visfatin (500-P222) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Visfatin. Western Blot: To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Visfatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P222BT-1MG will be provided as 2 x 500 μg

Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.
TRUSTED_SUSTAINABILITY

Specifications

Antigen NAMPT
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Biotin
Formulation PBS with no preservative
Gene NAMPT
Gene Accession No. P43490
Gene Alias 1110035O14Rik; AI314458; AI480535; DKFZp666B131; EC 2.4.2.12; hypothetical protein; MGC117256; NAmPRTase; NAMPT; nampt1; nampta; NAMPTP1; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase 1; nicotinamide phosphoribosyltransferase a; nicotinamide phosphoribosyltransferase pseudogene 1; PBEF; Pbef1; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; pre-B-cell colony-enhancing factor 1 precursor; VF; VISFATIN
Gene Symbols NAMPT
Host Species Rabbit
Immunogen E.coli-derived, 52.6 kDa Recombinant Human Visfatin.
Purification Method Antigen affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 10135
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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