CellSensor™ CellSensor™ NFκB-bla THP-1 Cell Line

Description

CellSensor™ NFκB-bla THP-1 Cell Line contains a beta-lactamase reporter gene under the control of the Nuclear Factor kappa B response element.

• The CellSensor™ NFκB-bla THP-1 cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor kappa B (NFκB) response element stably integrated into THP-1 cells.
• To obtain the cell line, the NFκB-bla construct was transduced into THP-1 cells by lentivirus.
• Subsequent flow cytometry was used to isolate cells responsive to Tumor Necrosis Factor alpha (TNFα).
• The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading time, and EC50 concentration of TNFα.
• The CellSensor™ NFκB-bla THP-1 cell line is responsive to TNFα and can be used to probe NFκB signaling pathways, including those involved in apoptosis, viral defense, cancer, inflammation, and autoimmune disease.
• This cell line can be adapted for high-throughput screening for agonists or antagonists with compound libraries.
• Candidate drugs can be tested for dose response against this cell line.

Specifications

• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Dividing Cells
• Content And Storage: CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
• Product Line: CellSensor™
• Quantity: 1 vial
• Target Entry: TNF alpha, NIK, TLR, I kappa B, IRAK, IKK, NF-kappa B pathway
• Type: Cell Line
• Cell Line: THP-1
• System Type: CellSensor™
• Druggable Target: Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.