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  6. NF-H Antibody (NE14) - Azide and BSA Free, Novus Biologicals™

NF-H Antibody (NE14) - Azide and BSA Free, Novus Biologicals™
SDP

Catalog No. NBP247834B Shop All R&D Systems Products
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NBP247834B 0.2 mg
NBP247834A 0.1 mg
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Catalog No. NBP247834B Supplier Novus Biologicals Supplier No. NBP2478340.2MG

Description

Mouse Monoclonal Antibody

NF-H Monoclonal antibody specifically detects Antigen in Chicken, Feline, Gerbil, Guinea Pig, Human, Mouse, Porcine, Primate, Rabbit, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry (Paraffin).

Specifications

Antigen NF-H
Applications Flow Cytometry, Immunohistochemistry (Paraffin), CyTOF
Classification Monoclonal
Clone NE14
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, CyTOF-ready
Formulation PBS with No Preservative
Gene Alias 200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H
Gene Symbols NEFH
Host Species Mouse
Immunogen Crude neurofilament preparation from porcine spinal cord
Molecular Weight of Antigen 200 kDa
Purification Method Protein A or G purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience
Primary or Secondary Primary
Gene ID (Entrez) 4744
Test Specificity This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Target Species Human, Mouse, Rat, Pig, Chicken, Feline, Gerbil, Guinea Pig, Primate, Rabbit
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1
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