NHE3/SLC9A3, p Ser552 Antibody (14D5), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 15 publications

Supplier:  Novus Biologicals NB11081529

Catalog No. NB11081529

Description

NHE3/SLC9A3 Monoclonal specifically detects NHE3/SLC9A3 in Human, Mouse, Rat, Porcine, Opossum, Primate, Rabbit samples. It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Knockout Validated, Knockdown Validated.

Specifications

• Antigen: NHE3/SLC9A3 (p Ser552)
• Classification: Monoclonal
• Concentration: 2 mg/mL
• Dilution: Western Blot 1-5 ug/ml, Simple Western 1:1000, Flow Cytometry reported in scientific literature (PMID 21255452), ELISA 1:100-1:2000, Immunohistochemistry 1:50-1:1000, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunoprecipitation 1:10-1:500, Knockout Validated reported in scientific literature (PMID 31276916), KnockDown Validated reported in scientific literature (PMID 31276916)
• Gene Symbols: SLC9A3
• Immunogen: Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunoassay, KnockDown
• Clone: 14D5
• Conjugate: Unconjugated
• Gene Alias: isoform 3, MGC126718, NHE3MGC126720, solute carrier family 9 (sodium/hydrogen exchanger), member 3, Solute carrier family 9 member 3
• Host Species: Mouse
• Molecular Weight of Antigen: 85 kDa
• Quantity: 0.1 mL
• Research Discipline: Lipid and Metabolism, Plasma Membrane Markers, Signal Transduction
• Gene ID (Entrez): 6550
• Target Species: Human, Mouse, Rat, Pig, Opossum, Primate, Rabbit
• Form: Purified

For Research Use Only