MP Biomedicals™ β-Nicotinamide Adenine Dinucleotide, Oxidized Form, MP Biomedicals™

Description

This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.

β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. It serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.

• Electron acceptor.
• β-NAD is a carrier for the hydride ion, forming b-NADH.
• Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase.
• Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH.
• Unlike b-NAD which has no absorbance at 340nm, b-NADH absorbs at 340nm (EmM = 6.22).
• The increase in absorbance at 340nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

Specifications

• Grade: Cell Culture
• Purity: ≥98%
• pH: 2 to 4 (1% aq soln)
• Content And Storage: 0°C, Desiccate
• Quantity: 100 mg
• Molecular Weight (g/mol): 663.4 (anhyd.)

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov