β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. It serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
- Electron acceptor.
- β-NAD is a carrier for the hydride ion, forming b-NADH.
- Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase.
- Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH.
- Unlike b-NAD which has no absorbance at 340nm, b-NADH absorbs at 340nm (EmM = 6.22).
- The increase in absorbance at 340nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.
|2–4 (1% aq soln)|
|Cell Culture Reagent|
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