MP Biomedicals™ β-Nicotinamide Adenine Dinucleotide, Oxidized, MP Biomedicals™

Description

Necessary for the catalytic reaction of certain enzymes. β-Nicotinamide Adenine Dinucleotide, Oxidized, MP Biomedicals is a pyridine nucleotide and biologically active form of nicotinic acid. β-NAD functions as an electron acceptor and occurs in

Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.

• Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols
• NAD usually acts as an electron acceptor, acting as a carrier for hydride ion, forming b-NADH which then serves as a hydrogen donor in the respiratory chain
  • Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase
  • Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH
  • Unlike b-NAD which has no absorbance at 340nm, b-NADH absorbs at 340nm (EmM = 6.22)
  • The increase in absorbance at 340nm with the formation of b-NADH is the basis for measurement of activity of many enzymes

Specifications

• Color: White
• Purity: ≥99%
• pH: 2 to 4 (1% aq soln)
• Content And Storage: -20°C, Store Desiccated
• Quantity: 100 mg
• Molecular Weight (g/mol): 663.4 (anhyd.)
• Form: Powder