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NNT1 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

$250.00 - $2175.00

Specifications

Antigen NNT1
Concentration 0.1-1.0 mg/mL
Applications ELISA, Western Blot
Classification Polyclonal
Conjugate Unconjugated
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
500P18650UG
Encompass_Preferred
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Invitrogen™
500P18650UG
50 μg
Each for $250.00
Only null left
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500P1861MG
Encompass_Preferred
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Invitrogen™
500P1861MG
1 mg
Each for $2,175.00
Only null left
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Description

Description

AA Sequence of recombinant protein: MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NNT-1/BCSF-3. Anti-Human NNT-1/BCSF-3-specific antibody was purified by affinity chromatography employing an immobilized Human NNT-1/BCSF-3 matrix. Sandwich ELISA: To detect Human NNT-1/BCSF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NNT-1/BCSF-3 (500-P186Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NNT-1/BCSF-3. Western Blot: To detect Human NNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NNT-1/BCSF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions

NNT-1/BCSF-3 is a neurotrophic factor with B-cell-stimulating capabilities. Expressed in the lymph nodes and spleen, NNT-1/BCSF-3 activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member b (LIFR-b) by binding and inducing the tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss, and B cell hyperplasia associated with increases in serum IgG and IgM.
Specifications

Specifications

NNT1
ELISA, Western Blot
Unconjugated
Rabbit
Human
Q9UBD9
23529
E.coli-derived Recombinant Human NNT-1/BCSF-3.
Antigen Affinity Chromatography
CLCF1
0.1-1.0 mg/mL
Polyclonal
Lyophilized
RUO
PBS with no preservative
b cell-stimulating factor 3; B-cell stimulating factor 3; B-cell stimulatory factor 3; B-cell-stimulating factor 3; Bsf3; BSF-3; Cardiotrophin-like cytokine factor 1; CISS2; Clc; CLCF1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT1; NNT-1; NNT-1/BSF-3; Novel neurotrophin-1; NR6
CLCF1
Primary
-20°C
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