NOV Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-271-8562	Invitrogen™ 500P257BT500UG	500 μg

Description

AA Sequence of recombinant protein: MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NOV. Anti-Human NOV-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hNOV by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NOV (500-P257) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNOV. Western Blot: To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNOV is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions 500-P257BT-1MG will be provided as 2 x 500 μg

NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm's tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell-surface integrins, and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities.

Specifications

• Antigen: NOV
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P48745
• Gene ID (Entrez): 4856
• Immunogen: E.coli-derived, 36.2 kDa Recombinant Human NOV.
• Purification Method: Antigen Affinity Chromatography
• Gene: CCN3

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: C130088N23Rik; CCN family member 3; CCN3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; IGFBP9; IGFBP-9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma overexpressed; nephroblastoma overexpressed gene; nephroblastoma overexpressed gene protein homolog; nephroblastoma-overexpressed gene protein homolog; NOV; NovH; protein NOV homolog
• Gene Symbols: CCN3
• Primary or Secondary: Primary
• Content And Storage: -20°C