Learn More
Invitrogen™ AcTEV™ Protease

Description
Includes
AcTEV™ Protease is supplied with a vial of 20X TEV buffer (1M Tris-HCl (pH 8.0), 10mM EDTA) and a vial of 100mM DTT.
An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity.
- Highly specific cleavage activity
- Enhanced enzyme stability for prolonged protease activity
- Activity over a broad temperature (4°C to 30°C) and pH (6.0 to 8.5) range
- Six-histidine sequence to facilitate its removal from the digested protein sample
- Greater than 85% single-band purity with no nonspecific protease contamination
- Application
- Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag
- This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant protein
- Purified from E. coli expressing the AcTEV™ Protease gene
- Unit definition
- One unit of AcTEV™ Protease cleaves 85% of a 3μg control substrate in 1 hr at 30°C
- Unit reaction conditions
- 50mM Tris-HCl (pH 8.0), 0.5mM EDTA, 1mM DTT, 3μg control substrate, and 1 unit enzyme in 30μL for 1 hr at 30°C AcTEV™ Protease is functionally tested for the absence of any nonspecific protease activity
GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Dry Ice

Specifications
Specifications
Concentration | 1,000 Units |
Content And Storage | AcTEV™ Protease is supplied with a vial of 20X TEV buffer [1 M Tris-HCl (pH 8.0), 10 mM EDTA] and a vial of 100 mM DTT. Store at -20° C. Guaranteed stable for 1 year when properly stored. |
Shipping Condition | Dry Ice |
Enzyme | TEV Protease |
Compatible Buffer | TEV Buffer |
Optimal Reaction Temperature | +4°C to +30°C |
Quantity | 1000 U |
Product Type | Protease |
Product Line | AcTEV™ |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.