Invitrogen™ Novex™ 10% Zymogram Plus (Gelatin) Protein Gels, 1.0 mm

Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol.

Supplier:  Invitrogen™ ZY00100BOX

Catalog No. ZY00100BOX

Description

Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10^-6 units of collagenase.

Formulation
Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and gelatin as the substrate. All have a 4% stacking gel.

Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.

Recommended buffers
Use Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC2675) with these gels. Novex Zymogram Renaturing Buffer (LC2670) and Novex Zymogram Developing Buffer (LC2671) are also recommended.

Recommended Storage

Store in refrigerator (2-8°C). Do not freeze.

Specifications

• For Use With (Equipment): Mini Gel Tank, XCell SureLock™ Mini-Cell
• Product Line: Novex
• Gel Type: Zymogram Gel
• No. of Wells: 10-well
• Sample Loading Volume: Up to 25 μL
• Shipping Condition: Wet Ice
• Thickness (Metric): 1.0 mm

• Gel Size: Mini
• Gel Percentage: 0.1
• Mode of Separation: Molecular Weight
• Quantity: 10 Gels/Box
• Separation Range: 30 to 150 kDa (Tris-Glycine Buffer)
• Temperature (Metric) Storage: Store at 2°C to 8°C. Do not freeze.
• Width (Metric): 8 cm