Horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues which can be conjugated to labeled biomolecules using Lightning-Link. The enzyme label can be visualized by chromogenic reactions; for example diaminobenzidine (DAB) in the presence of hydrogen peroxide (H202) is converted in to a water insoluble brown pigment. Other substrates which can be used to measure horseradish peroxidase activity include ABTS, TMB and TMBUS.
Store at -20°C. Avoid freeze-thaw cycles.
HRP Antibody Labeling Kit
Glass vial(s) of Lightning-Link mix (1, 3 or 5 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher reagent
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