NPM1, Mouse anti-Human, Clone: NPM1/1902, Novus Biologicals™

Catalog No.	Quantity
NB010495	100 μg
NB010496	20 μg

Catalog No. NB010495 Supplier Novus Biologicals Supplier No. NBP279739100UG

Description

Mouse Monoclonal Antibody

NPM1 Monoclonal specifically detects NPM1 in Human samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: NPM1
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Peptide Array
• Classification: Monoclonal
• Clone: NPM1/1902
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 1-2 ug/ml, ELISA 2-4 ug/ml, Immunohistochemistry 1-2 ug/ml, Immunohistochemistry-Paraffin 1-2 ug/ml, Protein Array
• Formulation: 10 mM PBS with 0.05% BSA
• Gene Accession No.: P06748, P06748
• Gene Alias: B23, MGC104254, NPMnucleophosmin/nucleoplasmin family, member 1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, nucleophosmin, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin
• Gene Symbols: NPM1
• Host Species: Mouse
• Immunogen: Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
• Purification Method: Protein A or G purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Cancer, Cell Cycle and Replication, Chromatin Research, DNA replication Transcription Translation and Splicing, Mitotic Regulators, Phospho Specific, Signal Transduction, Tumor Suppressors
• Primary or Secondary: Primary
• Gene ID (Entrez): 4869
• Test Specificity: This antibody recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM1). It is predominantly localized in the nucleus of cells in most tissues. NPM1 is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
• Target Species: Human
• Content And Storage: Store at 4°C.
• Form: Purified
• Isotype: IgG2b κ