NPM1, Mouse anti-Human, Clone: NPM1/3286, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP279740100UG

Catalog No. NB010497

Description

NPM1 Monoclonal specifically detects NPM1 in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: NPM1
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Western Blot 1-2 ug/ml, Flow Cytometry 1-2 ug/million cells, ELISA 2-4 ug/ml, Immunohistochemistry 1-2 ug/ml, Immunocytochemistry/Immunofluorescence 1-2 ug/ml, Immunohistochemistry-Paraffin 1-2 ug/ml, Protein Array
• Gene Accession No.: P06748, P06748
• Gene Symbols: NPM1
• Immunogen: Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
• Quantity: 100 μg
• Research Discipline: Apoptosis, Cancer, Cell Cycle and Replication, Chromatin Research, DNA replication Transcription Translation and Splicing, Mitotic Regulators, Phospho Specific, Signal Transduction, Tumor Suppressors
• Gene ID (Entrez): 4869
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: NPM1/3286
• Conjugate: Unconjugated
• Formulation: 10 mM PBS with 0.05% BSA
• Gene Alias: B23, MGC104254, NPMnucleophosmin/nucleoplasmin family, member 1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, nucleophosmin, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM1). It is predominantly localized in the nucleus of cells in most tissues. NPM1 is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
• Content And Storage: Store at 4°C.
• Isotype: IgG1 κ