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Nucleolin Antibody (364-5), Novus Biologicals™
SDP

Catalog No. NBP24461200 Shop All R&D Systems Products
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NBP24461200 0.02 mg
NBP24461201 0.1 mg
NBP24461202 0.2 mg
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Catalog No. NBP24461200 Supplier Novus Biologicals Supplier No. NBP2446120.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Nucleolin Monoclonal specifically detects Nucleolin in Human, Bovine (Negative), Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

Antigen Nucleolin
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone 364-5
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P19338
Gene Alias C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Gene Symbols NCL
Host Species Mouse
Immunogen Lysate of SU-DHL-1 Nuclei
Molecular Weight of Antigen 76 kDa
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 4691
Test Specificity Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Target Species Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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