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Nucleolin Antibody (NCL/902), Alexa Fluor™ 532, Novus Biologicals™
SDP

Catalog No. NP247860A32 Shop All R&D Systems Products
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NP247860A32 0.1 mL
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Catalog No. NP247860A32 Supplier Novus Biologicals Supplier No. NBP247860AF532
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Description

Mouse Monoclonal Antibody

Nucleolin Monoclonal specifically detects Nucleolin in Human, Bovine (Negative), Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen Nucleolin
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone NCL/902
Conjugate Alexa Fluor 532
Dilution Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence
Formulation 50 mM sodium borate with 0.05% sodium azide
Gene Alias C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Gene Symbols NCL
Host Species Mouse
Immunogen Recombinant full-length human Nucleolin protein (Uniprot: P19338)
Quantity 0.1 mL
Primary or Secondary Primary
Gene ID (Entrez) 4691
Test Specificity Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This Mouse Monoclonal Antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Target Species Human, Bovine, Mouse, Rat
Content And Storage Store at 4°C in the dark.
Isotype IgG1 κ
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