OPTIZYME T3 RNA Pol is a DNA-dependent RNA polymerase that catalyzes the 5'->3' synthesis of RNA from DNA.
Applications and Features:
- OPTIZYME T3 RNA Polymerase synthesizes unlabeled and labeled RNA that can be used for: (1) hybridization, (2) in vitro RNA translation, (3) RNase protection assays, (4) genomic DNA sequencing, (5) RNA splicing, and (6) studies of RNA secondary structure and RNA-protein interactions
- Incorporation of modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
5X OPTIZYME Transcription Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl2, 50mM DTT, 50mM NaCl, 10mM spermidine
Source: E. Coli cells with a cloned T3 RNA Polymerase gene
- Endo-, exodeoxyribonuclease and ribonuclease-free
- Functionally tested in in vitro transcription reaction
|T3 RNA Polymerase|
|E. coli cells with a cloned T3 RNA Polymerase gene|
|OPTIZYME™ Transcription Buffer 5X : Tris HCl 200mM (7.9 pH at 25°C), MgCl2 30mM, DTT 50mM, NaCl 50mM, Spermidine 10mM|
|Hybridization, in-vitro RNA translation, RNase protection assay, genomic DNA sequencing, RNA splicing and studies of RNA secondary structure and RNA-Protein interaction, quality control|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok