OPTIZYME™ T4 DNA Polymerase, Fisher BioReagents™

Description

OPTIZYME™ T4 DNA Pol is a template-dependent DNA polymerase that catalyzes 5'->3' synthesis from primed single-stranded DNA.

Applications and features:

• OPTIZYME T4 DNA Polymerase creates blunt DNA ends by filling in 5'-overhangs and/or removing 3'-overhangs
• Creation of blunt PCR products with 3'-dA overhangs
• Oligonucleotide-directed site-specific mutagenesis
• Synthesis of labeled DNA probes (by replacement reaction)
• Ligation-independent cloning of PCR products
• Active in buffers for OPTIZYME restriction enzymes, reverse transcriptases and T4 DNA ligase
• 3'->5' exonuclease activity
• Lacks 5'->3' exonuclease activity

Supplied with:
5X OPTIZYME T4 DNA Pol Buffer: 335mM Tris-HCl (pH 8.8 at 25°C), 33mM MgCl₂, 5mM DTT, 84mM (NH₄)₂SO₄

Source: E. Coli cells with a cloned gene 43 of bacteriophage T4

Quality Control:
Endodeoxyribonuclease-free

Specifications

• Concentration: 5 U/μL
• Color: Colorless
• pH: 7.5
• For Use With (Application): Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
• Storage Buffer: OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
• Content And Storage: -20°C
• Source: E. coli cells with a cloned gene 43 of bacteriophage T4
• Quantity: 100 U
• Product Type: T4 DNA Polymerase
• Form: Liquid

Safety and Handling

SDS