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OPTIZYME™ Terminal Deoxynucleotidyl Transferase, Fisher BioReagents™

Catalog No. BP81035 Shop All Fisherbrand Products
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1500 U
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BP81035 1500 U
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Catalog No. BP81035

Description

OPTIZYME™ TdT is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH group of DNA

Applications and Features:

  • OPTIZYME TdT produces synthetic homo- and heteropolymers
  • Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus
  • Oligodeoxyribonucleotide and DNA labeling
  • 5'-Rapid Amplification of cDNA Ends (5'-RACE)
  • In situ localization of apoptosis
  • Incorporation of modified nucleotides (e.g., fluorescein-, biotin-, aminoallyl-labeled nucleotides)

Supplied with:
5X OPTIZYME TdT Buffer: 1M potassium cacodylate, 125mM Tris, 0.05% (v/v) Triton X-100, 5mM CoCl2 (pH 7.2 at 25°C)

Source: E. Coli cells with a cloned gene encoding calf thymus terminal deoxynucleotidyl transferase

Quality Control:
Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free

TRUSTED_SUSTAINABILITY

Specifications

Concentration 20 U/μL
Color Colorless
pH 7.5
For Use With (Application) Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus, Oligodeoxyribonucleotide and DNA labeling, 5'-rapid amplification of cDNA Ends, in-situ localization of apoptosis incorporation of modified Nucleotides
Storage Buffer OPTIZYME™ TdT Buffer 5X : Potassium cacodylate 1M, Tris 125mM, Triton™ X-100 0.05%, CoCl2 5mM (7.2 pH at 25°C)
Content And Storage -20°C
Source E. coli cells with a cloned gene encoding calf thymus terminal deoxynucleotidyl transferase
Quantity 1500 U
Product Type Terminal Deoxynucleotidyl Transferase
Form Liquid
Product Title
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