Osteopontin/OPN Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 6 publications

Supplier:  Novus Biologicals NB6001043

Catalog No. NB6001043

Description

Osteopontin/OPN Polyclonal specifically detects Osteopontin/OPN in Human, Mouse samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Osteopontin/OPN
• Classification: Polyclonal
• Dilution: Western Blot 1:500-1:2000, ELISA 1:5000-1:20000, Immunohistochemistry 1:100-1:300, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin, N/A
• Gene Accession No.: p10451
• Gene Symbols: SPP1
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human Osteopontin/OPN protein, conjugated to KLH using maleimide. (Uniprot: P10451)
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Osteopontin/OPN is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: BNSP, Bone sialoprotein 1, MGC110940, Nephropontin, osteopontin, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, uropontin
• Host Species: Rabbit
• Molecular Weight of Antigen: 35.42 kDa
• Quantity: 0.2 mL
• Research Discipline: Cellular Markers
• Gene ID (Entrez): 6696
• Target Species: Human, Mouse, Rat, Pig, Canine
• Form: Antisera

For Research Use Only