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  6. Invitrogen™ OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), PE-Cyanine7, eBioscience™, Invitrogen™ 

Invitrogen™ OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), PE-Cyanine7, eBioscience™, Invitrogen™

Catalog No. p-7090440
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25 μg
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Catalog No. Quantity
50-245-749 25 μg
50-245-750 100 μg
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Catalog No. 50-245-749 Supplier Invitrogen™ Supplier No. 25574380
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Description

Mouse Monoclonal Antibody

Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo. Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal antibody specifically detects OVA257-264 (SIINFEKL) peptide bound to H-2Kb in Mouse samples. It is validated for Flow Cytometry.
TRUSTED_SUSTAINABILITY

Specifications

Antigen OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Applications Flow Cytometry
Classification Monoclonal
Clone eBio25-D1.16 (25-D1.16)
Concentration 0.2 mg/mL
Conjugate PE-Cyanine7
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb
Host Species Mouse
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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For Research Use Only.

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