p21/CIP1/CDKN1A Antibody (WA-1), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP229463

Catalog No. NBP229463

Description

p21/CIP1/CDKN1A Monoclonal antibody specifically detects p21/CIP1/CDKN1A in Human, Mouse, Chimpanzee, Monkey samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

• Antigen: p21/CIP1/CDKN1A
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Western Blot, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry 0.5-1ug/ml, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Gene Alias: CAP20cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDKN1melanoma differentiation associated protein 6, CIP1WAF1CDK-interaction protein 1, cyclin-dependent kinase inhibitor 1A (p21, Cip1), MDA6, MDA-6, Melanoma differentiation-associated protein 6, p21, p21CIP1, p21Cip1/Waf1, PIC1, SDI1DNA synthesis inhibitor, wild-type p53-activated fragment 1
• Host Species: Mouse
• Molecular Weight of Antigen: 21 kDa
• Quantity: 0.1 mg
• Research Discipline: Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific
• Gene ID (Entrez): 1026
• Target Species: Human, Mouse, Chimpanzee, Monkey
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: WA-1
• Conjugate: Unconjugated
• Gene Accession No.: P38936
• Gene Symbols: CDKN1A
• Immunogen: Human recombinant p21/CIP1/CDKN1A protein (Uniprot: P38936 )
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, monoclonal antibody WA-1 is useful either as a solid phase or for detection of p21 protein.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only