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  6. p27/Kip1 Antibody (DCS-72.F6), Novus Biologicals™

p27/Kip1 Antibody (DCS-72.F6), Novus Biologicals™
SDP

Catalog No. NBP24449600 Shop All R&D Systems Products
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NBP24449600 0.02 mg
NBP24449601 0.1 mg
NBP24449602 0.2 mg
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Catalog No. NBP24449600 Supplier Novus Biologicals Supplier No. NBP2444960.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

p27/Kip1 Monoclonal specifically detects p27/Kip1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen p27/Kip1
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone DCS-72.F6
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P46527
Gene Alias CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
Gene Symbols CDKN1B
Host Species Mouse
Immunogen Mouse recombinant p27 protein
Molecular Weight of Antigen 25.5 kDa
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Prostate Cancer, Tumor Suppressors
Primary or Secondary Primary
Gene ID (Entrez) 1027
Test Specificity Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Target Species Human, Mouse, Rat, Monkey
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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