P2Y12 Receptor, Rabbit anti-Rat, Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
ABS148725UG	MilliporeSigma ABS148725UG	25 μg
ABS1487100	MilliporeSigma ABS1487100UG	100 μg

Description

Anti-P2Y12 Receptor, Cat. No. ABS1487, is a rabbit polyclonal antibody that detects P2Y purinoceptor 12 and has been tested for Immunohistochemistry (Paraffin) and Western Blotting.

P2Y purinoceptor 12 (UniProt: Q9H244; also known as P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999) is encoded by the P2RY12 (also known as HORK3) gene (Gene ID: 64805) in human. P2Y12 is a multi-pass metabotropic receptor that is highly expressed in the platelets. Lower expression levels have been observed in the brain, lung, and adrenal glands. P2Y12 serves as a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. It is not activated by UDP and UTP. It is required for normal platelet aggregation and blood coagulation. It is the predominant receptor involved in the ADP-stimulated activation of the glycoprotein IIb/IIIa receptor. Activation of the glycoprotein IIb/IIIa receptor results in enhanced platelet degranulation and thromboxane production, and prolonged platelet aggregation. P2Y12 receptor has also been reported to be expressed on microglia and is required to neuropathic pain following peripheral nerve injury. P2Y12 has seven transmembrane, four cytoplasmic, and four extracellular domains. The transmembrane helices are somewhat tilted and/or kinked. Agonist binding promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. Mutations in P2RY12 gene have been linked to bleeding disorders resulting from severe impairment of platelet response to ADP resulting in defective platelet aggregation. (Ref.: Burnstock G. (2009). Curr. Pharm. Des. 15(15);1717-35; Damman, P., et al. (2012). J. Thromb. Thrombolysis. 33(2); 143-153).

Specifications

• Antigen: P2Y12 Receptor
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Rat, Human
• Gene Alias: P2Y purinoceptor 12;ADP-glucose receptor;ADPG-R;P2T(AC);P2Y(AC);P2Y(cyc);P2Y12 platelet ADP receptor;P2Y(ADP);SP1999
• Gene Symbols: P2RY12;HORK3
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Molecular Weight of Antigen: ∽50 kDa observed; 39.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250-1,000 dilution from a representative lot detected P2Y12 Receptor in human cerebral cortex and human tonsil tissue sections.Western Blotting Analysis: 1μg/mL from a representative lot detected P2Y12 Receptor in human platelet cell lysate.
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Signaling
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene ID (Entrez): NP_073625
• Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the internal region of human P2Y purinoreceptor 12.
• Purification Method: Affinity Purified
• Test Specificity: This rabbit polyclonal antibody specifically detects P2Y12 receptor. It targets an epitope with in 13 amino acids from the extracellular domain.