p53 Antibody (PAb 240) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 31 publication

Supplier:  Novus Biologicals NB200103SS

Catalog No. NB200103SS

Description

p53 Monoclonal specifically detects p53 in Human, Mouse, Rat, Yeast, Xenopus (Negative) samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-ready.

Specifications

• Antigen: p53
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:1000-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:250-1:500, Immunocytochemistry/Immunofluorescence 5 ug/ml. Use reported in scientific literature (PMID 1394225), Immunoprecipitation 10ug/mg, Immunohistochemistry-Paraffin 1:250-1:500, Immunohistochemistry-Frozen reported in multiple pieces of scientific literature, Flow (Intracellular), CyTOF-ready
• Gene Symbols: TP53
• Immunogen: Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).
• Quantity: 0.025 mL
• Research Discipline: Apoptosis, Cancer, Cell Cycle and Replication, Checkpoint signaling, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, Hypoxia, p53 Pathway, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors
• Gene ID (Entrez): 7157
• Target Species: Human, Mouse, Rat, Yeast, Xenopus (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Clone: PAb 240
• Conjugate: Unconjugated
• Gene Alias: Antigen NY-CO-13, FLJ92943, LFS1TRP53, p53, p53 tumor suppressor, P53cellular tumor antigen p53, Phosphoprotein p53, transformation-related protein 53, tumor protein p53, Tumor suppressor p53
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This monoclonal recognizes both mutant forms and wild-type human p53 under denaturing conditions. It does not recognize nondenatured wild type p53. In nondenaturing conditions (IP) it only recognizes mutant p53.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only