p73 Antibody (5B429), Alexa Fluor™ 350, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP224737AF350

Catalog No. NP224737A30

Description

p73 Monoclonal specifically detects p73 in Human, Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular).

Specifications

• Antigen: p73
• Classification: Monoclonal
• Conjugate: Alexa Fluor 350
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: TP73
• Immunogen: This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
• Primary or Secondary: Primary
• Test Specificity: p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674/ IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 5B429
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular)
• Gene Alias: p53-related protein, P73p53-like transcription factor, TA p73, TAp73, tumor protein p73
• Host Species: Mouse
• Quantity: 0.1 mL
• Gene ID (Entrez): 7161
• Target Species: Human, Mouse, Rat
• Isotype: IgG1 κ