alpha Tubulin Antibody (YL1/2) - BSA Free, Novus Biologicals™

Rat Monoclonal Antibody has been used in 38 publications

Supplier:  Novus Biologicals NB600506

Catalog No. NB600506

Description

alpha Tubulin Monoclonal specifically detects alpha Tubulin in Human, Mouse, Rat, Avian, C. elegans, Drosophila, Invertebrate, Mammal, Monkey, Primate, Yeast samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Functional, Radioimmunoassay.

Specifications

• Antigen: alpha Tubulin
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:5000-1:10000, Flow Cytometry 2-5 ug/0.1x10^6 cells, ELISA 1:100-1:1000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000-1:10000. Use reported in scientific literature (PMID 28001364), Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-Frozen 1:200, Functional reported in scientific literature (PMID 31358662), Radioimmunoassay
• Gene Symbols: TUBA1A
• Immunogen: This alpha Tubulin Antibody (YL1/2) was developed against full length native protein (purified) (S. cerevisiae).
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Human, mouse, rat, monkey, S. cerevisiae, S. pombe, Slime moulds, Allium and birds. Expected to react with most eukaryotes due to sequence identity. Drosophila reactivity reported in scientific literature (PMID: 24019759) Other species have not been tested.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Functional Assay, Radioimmune Assays (RIA)
• Clone: YL1/2
• Conjugate: Unconjugated
• Gene Alias: Alpha-tubulin 3, B-ALPHA-1, FLJ25113, hum-a-tub1, hum-a-tub2, LIS3, TUBA3, tubulin alpha-1A chain, Tubulin alpha-3 chain, Tubulin B-alpha-1, tubulin, alpha 1a, tubulin, alpha 3, tubulin, alpha, brain-specific
• Host Species: Rat
• Molecular Weight of Antigen: 50 kDa
• Quantity: 0.1 mL
• Research Discipline: Cell Biology, Cell Cycle and Replication, Cytoskeleton Markers, Loading Controls, Neuroscience
• Gene ID (Entrez): 7846
• Target Species: Human, Mouse, Rat, Avian, C. elegans, Drosophila, Invertebrate, Mammalia, Monkey, Primate, Yeast
• Form: Purified

For Research Use Only