pan Actin Mouse, Clone: HHF35, Novus Biologicals™

Catalog No.	Quantity
NBP234230A	0.1 mg
NBP234230F	0.02 mg
NBP234230B	0.2 mg

Catalog No. NBP234230A Supplier Novus Biologicals Supplier No. NBP2342300.1MG

Description

Mouse Monoclonal Antibody

pan Actin Monoclonal specifically detects pan Actin in Canine, Chicken, Feline, Human, Mouse, Rabbit, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Simple Western, Western Blot.

Specifications

• Antigen: pan Actin
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Simple Western, Western Blot
• Classification: Monoclonal
• Clone: HHF35
• Conjugate: Unconjugated
• Dilution: Western Blot 0.5-1.0ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Accession No.: P62736
• Gene Alias: ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3
• Gene Symbols: ACTA1
• Host Species: Mouse
• Immunogen: SDS extract of human myocardium.
• Purification Method: Protein A purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 58
• Test Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Target Species: Canine, Chicken, Feline, Human, Mouse, Rabbit, Rat
• Content And Storage: Store at 4C.
• Form: Purified
• Isotype: IgG1 κ