pan Actin Mouse, Clone: HHF35, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234230F	Novus Biologicals NBP2342300.02MG	0.02 mg
NBP234230A	Novus Biologicals NBP2342300.1MG	0.1 mg
NBP234230B	Novus Biologicals NBP2342300.2MG	0.2 mg

Description

Actin (Muscle Specific) Monoclonal specifically detects Actin (Muscle Specific) in Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: pan Actin
• Dilution: Western Blot 0.5-1.0ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3
• Gene Symbols: ACTA1
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

• Clone: HHF35
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit
• Gene Accession No.: P62736
• Gene ID (Entrez): 58
• Immunogen: SDS extract of human myocardium.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.