PCNA Antibody (PC10), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 35 publications

Supplier:  Novus Biologicals NB500106

Catalog No. NB500106

Description

PCNA Monoclonal specifically detects PCNA in Human, Mouse, Rat, Porcine, Chicken, Drosophila, Fish, Marsupial, Primate, Rabbit, Yeast, Zebrafish samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating.

Specifications

• Antigen: PCNA
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Western Blot 1:2000, Simple Western 1:200, Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 26883631), Flow Cytometry 1:10-1:1000, ELISA reported in scientific literature, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:1000-1:2000. Use reported in scientific literature (PMID 22068968), Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:500-1:1000, Immunohistochemistry-Frozen 1:10-1:500, Immunohistochemistry Free-Floating reported in scientific literature (PMID 27212918), Chromatin Immunoprecipitation (ChIP) 1:10-1:500
• Gene Alias: cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
• Host Species: Mouse
• Molecular Weight of Antigen: 30 kDa
• Quantity: 0.1 mL
• Research Discipline: Autophagy, Base Excision Repair, Cellular Markers, DNA Repair, Loading Controls
• Gene ID (Entrez): 5111
• Target Species: Human, Mouse, Rat, Pig, Chicken, Drosophila, Fish, Marsupial, Primate, Rabbit, Yeast, Zebrafish
• Form: Purified

• Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry
• Clone: PC10
• Conjugate: Unconjugated
• Gene Accession No.: P12004
• Gene Symbols: PCNA
• Immunogen: Protein A-rat PCNA fusion obtained from pC2T
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Specific for PCNA p36 protein expressed at high levels in proliferating cells.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a κ

For Research Use Only