PD-ECGF/Thymidine Phosphorylase Antibody (SPM322), DyLight 488, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP254403G

Catalog No. NBP254403G

Description

PD-ECGF/Thymidine Phosphorylase Monoclonal specifically detects PD-ECGF/Thymidine Phosphorylase in Human, Mouse, Rat samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: PD-ECGF/Thymidine Phosphorylase
• Classification: Monoclonal
• Conjugate: DyLight 488
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: TYMP
• Immunogen: Recombinant full-length human PD-ECGF/Thymidine Phosphorylase protein (Uniprot: P19971)
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Clone: SPM322
• Dilution: Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin
• Gene Alias: EC 2.4.2, EC 2.4.2.4, ECGF, ECGF1TP, endothelial cell growth factor 1 (platelet-derived), Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PDECGF, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase, thymidine phosphorylase
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Research Discipline: Angiogenesis
• Gene ID (Entrez): 1890.0
• Target Species: Human, Mouse, Rat
• Isotype: IgG1 κ