PEG, Mouse anti-All, Clone: 6.3, Millipore Sigma™

Catalog No.	Quantity
MABS1966100	100 μg
MABS196625	25 μg

Catalog No. MABS1966100 Supplier MilliporeSigma Supplier No. MABS1966100UG

Description

Mouse Monoclonal Antibody

Anti-PEG, clone 6.3, Cat. No. MABS1966, is a mouse monoclonal antibody that detects PEG-conjugated proteins and it hs been tested for use in ELISA.

Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. Clone 6.3 displays high affinity and binds the PEG backbone and can be employed as a detection antibody for the quantification of PEG. It binds to a smaller epitope of the PEG backbone when compared to other antibodies. Since it binds to a smaller PEG epitope (∽750 Da), it is not compatible with detergents that contain an ethylene oxide backbone, such as Tween 20. Instead 0.05% CHAPS is a more suitable detergent when using this antibody.

Specifications

• Antigen: PEG
• Applications: ELISA
• Classification: Monoclonal
• Clone: 6.3
• Conjugate: Unconjugated
• Dilution: ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
• Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
• Gene Alias: Polyethylene Glycol
• Host Species: Mouse
• Immunogen: PEG-conjugated to E.coli beta-glucuronidase.
• Purification Method: Protein A purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Test Specificity: Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
• Target Species: All species
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ