PEG, Mouse anti-All, Clone: 6.3, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABS1966100UG

Catalog No. MABS1966100

Description

Anti-PEG, clone 6.3, Cat. No. MABS1966, is a mouse monoclonal antibody that detects PEG-conjugated proteins and it hs been tested for use in ELISA.

Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. Clone 6.3 displays high affinity and binds the PEG backbone and can be employed as a detection antibody for the quantification of PEG. It binds to a smaller epitope of the PEG backbone when compared to other antibodies. Since it binds to a smaller PEG epitope (∽750 Da), it is not compatible with detergents that contain an ethylene oxide backbone, such as Tween 20. Instead 0.05% CHAPS is a more suitable detergent when using this antibody.

Specifications

• Antigen: PEG
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
• Host Species: Mouse
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: All species
• Form: Purified

• Applications: ELISA
• Clone: 6.3
• Dilution: ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
• Gene Alias: Polyethylene Glycol
• Immunogen: PEG-conjugated to E.coli beta-glucuronidase.
• Quantity: 100 μg
• Research Discipline: Signaling
• Test Specificity: Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ