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PEG, Mouse anti-All, Clone: AGP4, Millipore Sigma™

Catalog No. MABS196425 Shop All MilliporeSigma Products
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MABS196425 25 μg
MABS1964100 100 μg
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Catalog No. MABS196425 Supplier MilliporeSigma Supplier No. MABS196425UG
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Description

Mouse Monoclonal Antibody

Anti-PEG, clone AGP4, Cat. No. MABS1964, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Western Blotting, and Immunohistochemistry.

Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. PEG is considered as a non-immunogenic moiety and has been widely tested for its ability to protect proteins from the host s immune system. This antibody (Clone AGP4) binds to the PEG backbone and is suitable for capture of PEGylated compounds in ELISA as well as the detection of PEGylated molecules. It is suitable for sensitive ELISA applications where AGP4 can be used as a capture antibody. Note: Tween-20 should be used in wash buffers for this antibody combination.
TRUSTED_SUSTAINABILITY

Specifications

Antigen PEG
Applications ELISA, Immunohistochemistry, Western Blot
Classification Monoclonal
Clone AGP4
Conjugate Unconjugated
Dilution Immunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
Formulation Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
Gene Alias Polyethylene Glycol
Host Species Mouse
Immunogen Polyethylene glycol-conjugated to E.coli beta-glucuronidase.
Purification Method Purified by ion-exchange chromatography
Quantity 25 μg
Regulatory Status RUO
Research Discipline Signaling
Primary or Secondary Primary
Test Specificity Clone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
Target Species All species
Content And Storage Stable for 1 year at 2-8°C from date of receipt.
Form Purified
Isotype IgM κ
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