MP Biomedicals™ Pepsin, MP Biomedicals™

Description

Used to digest proteins and in the preparation of Fab fragments from antibodies. Pepsin, MP Biomedicals is an acid protease that contains a proteolytic enzyme with a in.lcathepsinin.l component that has milk curdling activity. It has a broad range of substrate

Pepsin is the major proteolytic enzyme produced in the stomach and is used to digest proteins. It is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.

Pepsin digests proteins through the cleavage of interior peptide linkages. It hydrolyzes peptide bonds, not amide or ester linkages.

• Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond
• Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond
• Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues
• Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Specifications

• Color: Yellowy Tan
• pH: Optimum pH 1 to 3.5, unstable above 6.0
• For Use With (Application): Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.
• Content And Storage: -20°C, Desiccate
• Source: Porcine Stomach Mucosa
• Quantity: 250 mg
• Molecular Weight (g/mol): ∽35,000
• Form: Powder

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov