MP Biomedicals™ Peroxidase, MP Biomedicals™

Description

Used in biochemistry applications such as western blots, ELISA and immunohistochemistry. Peroxidase, MP Biomedicals is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is an essentially

Horseradish Peroxidase (HRP) is a single chain polypeptide containing four disulfide bridges. At least seven isozymes exist. When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric or luminescent derivative of the labeled molecule, allowing quantification. It is a glycoprotein containing 18% carbohydrate.

• The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine and galactosamine, depending upon the specific isozyme
• Its molecular weight (∼44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (∼700 Daltons), and carbohydrate (∼9,400 Daltons)
• Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant
• Known inhibitors are sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions

HRP is used in biochemistry applications. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein.

• It is commonly used to determine amounts of glucose and peroxides in solution. It has been used in an aspergillus fumigatus rapid susceptibility assay
• Can be conjugated to antibodies by several different methods
• Conjugation methods include glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers
• It is the most desired label for antibodies, since it is the smallest and most stable of the three most popular enzyme labels (HRP, β-galactosidase, and alkaline phosphatase) and its glycosylation leads to lower non-specific binding
• A review of glutaraldehyde and periodate conjugation methods has been published
• HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens
• It is also useful for tracing neural connections, including motor and sensory innervation of peripheral organs, and connections of peripheral nerves, ganglia and individual dorsal roots

Specifications

• Color: Reddish Brown
• pH: Optimal pH range: 6.0 to 6.5 (activity at pH 7.5 is 84% of the maximum). The enzyme is most stable in the pH range of 5.0 to 9.0. (Lit.)
• Content And Storage: 0°C
• Quantity: 50 kU
• Molecular Weight (g/mol): 44,000 (Polypeptide=33,890; hemin+calcium=∽700; carbohydrate=∼9365) (Lit.)
• Form: Crystalline Powder