Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

MP Biomedicals™ Peroxidase, MP Biomedicals™

Used in biochemistry applications such as western blots, ELISA and immunohistochemistry. Peroxidase, MP Biomedicals is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is an essentially

$333.00 - $1698.00

Specifications

pH Optimal pH range: 6.0–6.5 (activity at pH 7.5 is 84% of the maximum). The enzyme is most stable in the pH range of 5.0–9.0. (Lit.)
Format Crystalline Powder
Molecular Weight 44,000 (Polypeptide=33,890; hemin+calcium=∽700; carbohydrate=∼9365) (Lit.)
Storage Requirements Store at 0°C.
View More Specs

Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
MP215003805 View Documents MP Biomedicals™
0215003805
5kU Each for $333.00
Add to cart
 
MP215003825 View Documents MP Biomedicals™
0215003825
25kU Each for $1,062.00
Add to cart
 
MP215003850 View Documents MP Biomedicals™
0215003850
50kU Each for $1,698.00
Add to cart
 
Description

Description

Horseradish Peroxidase (HRP) is a single chain polypeptide containing four disulfide bridges. At least seven isozymes exist. When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric or luminescent derivative of the labeled molecule, allowing quantification. It is a glycoprotein containing 18% carbohydrate.

  • The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine and galactosamine, depending upon the specific isozyme
  • Its molecular weight (∼44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (∼700 Daltons), and carbohydrate (∼9,400 Daltons)
  • Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant
  • Known inhibitors are sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions

HRP is used in biochemistry applications. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein.

  • It is commonly used to determine amounts of glucose and peroxides in solution. It has been used in an aspergillus fumigatus rapid susceptibility assay
  • Can be conjugated to antibodies by several different methods
    • Conjugation methods include glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers
    • It is the most desired label for antibodies, since it is the smallest and most stable of the three most popular enzyme labels (HRP, β-galactosidase, and alkaline phosphatase) and its glycosylation leads to lower non-specific binding
    • A review of glutaraldehyde and periodate conjugation methods has been published
  • HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens
  • It is also useful for tracing neural connections, including motor and sensory innervation of peripheral organs, and connections of peripheral nerves, ganglia and individual dorsal roots
Specifications

Specifications

Optimal pH range: 6.0–6.5 (activity at pH 7.5 is 84% of the maximum). The enzyme is most stable in the pH range of 5.0–9.0. (Lit.)
Crystalline Powder
44,000 (Polypeptide=33,890; hemin+calcium=∽700; carbohydrate=∼9365) (Lit.)
Store at 0°C.
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit