Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media

Description

Used for capture and intermediate purification requiring low to medium hydrophobicity

• Base matrix derivatized via uncharged, chemically-stable ether linkages
• Two levels of substitution help find optimal selectivity and binding capacity for given application
• Hydrophilic nature of the base matrix ensures low levels of non-specific binding
• Hydrophobic interaction chromatography (HIC) media composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages
• Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development
• Degree of substitution for low sub HIC media is ∽25μmol phenyl/mL gel
• Degree of substitution for high sub HIC media is ∽40μmol phenyl/mL gel

Specifications

• Quantity: 25 mL
• For Use With (Application): For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity
• Stability: Stable in commonly used aqueous Buffers: 1 M NaOH, 3 M Ammonium Sulphate, 30% Isopropanol, 70% Ethanol, 10% Ethylene Glycol, 0.5% SDS, 6 M Guanidine-Hydrochloride, 8 M Urea
• Storage Requirements: 4°C to 30°C
• Product Type: Phenyl Sepharose 6 Fast Flow Media (low sub)
• Flow Rate: 250 to 400 cm/Hoμr
• Particle Size: 45 to 165 μm
• Matrix: 6% Cross-Linked Agarose
• pH Range: 3 to 13
• Storage Buffer: 20% Ethanol
• Ligand Type: Phenyl