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Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media

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Quantity:
25 mL
200 mL
Product Type:
Phenyl Sepharose 6 Fast Flow Media (high sub)
Phenyl Sepharose 6 Fast Flow Media (low sub)
Description
- Base matrix derivatized via uncharged, chemically-stable ether linkages
- Two levels of substitution help find optimal selectivity and binding capacity for given application
- Hydrophilic nature of the base matrix ensures low levels of non-specific binding
- Hydrophobic interaction chromatography (HIC) media composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages
- Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development
- Degree of substitution for low sub HIC media is ∽25μmol phenyl/mL gel
- Degree of substitution for high sub HIC media is ∽40μmol phenyl/mL gel
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Specifications
Specifications
Quantity | 200 mL |
For Use With (Application) | For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity |
Stability | Stable in commonly used aqueous Buffers: 1 M NaOH, 3 M Ammonium Sulphate, 30% Isopropanol, 70% Ethanol, 10% Ethylene Glycol, 0.5% SDS, 6 M Guanidine-Hydrochloride, 8 M Urea |
Storage Requirements | 4°C to 30°C |
Product Type | Phenyl Sepharose 6 Fast Flow Media (high sub) |
Flow Rate | 250 to 400 cm/Hoμr |
Particle Size | 45 to 165 μm |
Matrix | 6% Cross-Linked Agarose |
pH Range | 3 to 13 |
Storage Buffer | 20% Ethanol |
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