Thermo Scientific™ Phire Plant Direct PCR Master Mix, 1,250 reactions PROMO

Designed to amplify DNA directly from plant samples.

Supplier:  Thermo Scientific™ F160L

Catalog No. F160LW25

Description

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.Suitable for 1250 reactions.

Features

• Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
• Available Dilution & Storage protocol allows several PCR reactions from one sample
• Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
• Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Notes

• Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

• Concentration: 2X
• GC-Rich PCR Performance: Medium
• Polymerase: Phire Hot Start II DNA Polymerase
• Product Type: Direct PCR Master Mix
• Shipping Condition: Wet or Dry Ice
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Plant

• Content And Storage: 2X Phire Plant Direct PCR Master Mix, 10 x 1.25 mL, Dilution Buffer, 2 x 5 mL, 25 μM Control Primer Mix, 40 μL, Nuclease-free Water, 10 x 1.25 mL, O'GeneRuler Express DNA Ladder, 50 μg (Sufficient for 100 Applications). Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Quantity: 1250 Reactions
• For Use With (Application): Genotyping, Hot-start PCR
• No. of Reactions: 1250 Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA